The problem with this is that the 'high' side battery (running 12V) can still find ground through the voltage regulator.
Steven (RWH) pointed this out earlier in the thread.........."connection to the 14v tap off the regulator can and will also act as a ground when the ground is disconnected allowing the truck to be cranked over but on 12v only".
I would recommend cutting off the negative of both batteries, ie interconnect on low side battery and ground on high side battery.
If you do that there is no way for anything to get power.
But if you cut off the positive side on the first battery it will also accomplish same thing and isolate the battery bank.
One of the first things a student in electronics/electrical 101 learns is that in order to have current flow one must have a path to and from the source.
Because the vehicle is not running the only source is the batteries.
A 12V battery in itself is a simple series circuit, 6 cells connected in series, removing any of the cells OR removing ether the positive or negative terminal will prevent any current flow through the battery.
The circuit below represents what the OP was talking about, since he has effectively removed the negative terminal from the low side battery and the positive terminal from the high side battery no current can flow through ether battery, effectively isolating all energy from the vehicle circuits.
50 years experience tells me the following is the preferred switch setup, I use the little weeny battery switches that some think are adequate for a main switch for the 12V and a 1500A Hella switch for the negative (24V) disconnect.
This way I can first isolate the 12V equipment then the 24V volt equipment with zero chance of reverse polarity of the 12V equipmewnt IE
12V OFF first and 12V ON last

There is no need to disconnect the large lead between the batteries, depending on if the 12V equipment is connected to the positive terminal of the low side battery or the negative terminal of the high side battery (works ether way) you can leave yourself unprotected.